Analytical study on random mobility and chemokinesis of human granulocytes in the agarose plate.

Toxo-OKA, T., NAKAYAMA, M. and MATSUMOTO, S. Analytical Study on Random Mobility and Chemokinesis of Human Granulocytes in the Agarose Plate. Tohoku J. exp. Med., 1979, 127 (3), 229-235-The minimum essential factors which are necessary for random mobility and chemokinesis of human granulocytes in agarose plate induced by E. coli-derived chemotactic factor were studied in order to compare the essential nature of mobility between them. There were no differences in random mobility and chemokinesis among the media of TC-199 containing or not containing heat inactivated fetal calf serum and Hanks solution. On the other hand, granulocytes in PBS showed no random mobility and a reduced chemokinesis was seen. Further precise analysis revealed that the minimum essential factors for random mobility and chemokinesis are phosphate buffered saline, Ca++, Mg++ and glucose. The necessity for exogenous glucose suggested that intracellular glycolysis is the source of energy for chemokinesis as well as for random mobility. On the other hand, actinomycin D, a nucleic acid synthesis inhibitor, failed to show any suppressive effect on both types of mobili ty. It was also shown that ehemokinesis as well as random mobility was highly sensitive to culture temperature. From these results, we concluded that the nature of mobility in chemokinesis is the same as that in random mobility.granulocyte; chemokinesis; random mobility; chemical factors